Hi everyone. We're continuing the comparative genomic analysis section in PATRIC. What I'm going to show you how to do now is to create a genome group. Earlier, we showed you how you could start with a list of unique genome identifiers and use the global search function to return a list of genomes. These are the same genomes that we use in the other instructional video. Creating a genome group is really easy in PATRIC. Notice this checkbox here in the first column across from genome name, if I go ahead and click that, it's going to auto select all of the 39 genomes on this page. You notice when I did that, it populated this vertical green bar with possible bounced dream functions or actions that you could do. I could download the data in the table, I could copy it, I could see all the genomes. Pretty much a view like I have here, or I could create a group, or I can remove individual genomes from the selection. What we want to do is to create a group. Let's click on this icon. Now, you may have groups created in PATRIC, but we want to create a new group. Let's call this whatever you want. I'm going to call it 39 Brucella genomes. Once I've done that, you notice that the blue button is highlighted, which means that I can add that group. I'm going to add the group. Notice once I do that, a little pop-up box comes here to tell me that group successfully created. If I want to double-check to make sure that that group is there, I can go into workspaces, click on that, and then click on Genome Groups. I have a ton of genome groups. You can see when I started it long time ago, in the table in PATRIC, you can resort it by clicking on the table heads. To get to my most recently created one, I'm going to click on created, and there it is. It says 39 Brucella genomes. I click on that, and then if I want to verify that everything is there, once again, look at this green bar with the downstream actions, then you click on the genome group. It's going to overwrite the page. This is summing all the data for all the genomes in that group that I want to check those genomes to see who they are, and it looks like those are my 39. Now that's the way you create a genome group in PATRIC, very easy, dead easy. Let me click on one here. This Brucella ceti str Cudo. This time I want to go to the genome page. I can add this genome to a group right here. You can add things to an existing group if you want. Well, actually could look at the most recent group, and there it is, even now it's a member of it, and I can say add that genome and it's added that one item. That's the way that you create a genome group in PATRIC. We're going to use this group in downstream exercises with the Protein Family Sorter. We're going to step through each of these in our comprehensive genome analysis series. But at any time if you have questions, if you don't understand something and you need some help, up here on the Help tab, we have tutorials, we have webinars, instructional videos, but also if you click on Provide Feedback, it will give you a way to say I can't create a group, I can't do something. This will send the message directly to me and I'll get in contact with you and help you. Thanks for listening and keep watching the series so you can learn how to use the Protein Family Sorter and thank you for using PATRIC. Are you ready for your second assignment? I want you to create two genome groups. One, that have the Unicycler strategy for those hybrid reads that we talked about earlier, and the other, that use Canu as the assembly strategy. So they'll be different, Racon and Pilon iterations among those, but I want you to have those two specific groups. You can use global search, you can go in through my genomes, or you can go in through the jobs result page and drill down. Good luck.
