About this Course
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This course aims to provide the basic knowledge about extracellular vesicles (EV) a generic term including exosomes, microvesicles, microparticles, ectosomes, oncosomes, prostasomes, and many others. It covers areas such as EV history, nomenclature, biogenesis, EV cargo as well as the release and uptake mechanisms, collection and processing prior to isolation, different isolation methods, characterization and quantification techniques. This course is divided into five modules. Module 1 is an introduction to the field and will cover the nomenclature and the history of EVs. Module 2 will focus on the biogenesis, release and uptake mechanisms of EVs as well as the different EV cargos (RNA, protein, lipids). In Module 3, we will focus on the collection and processing of cell culture media and body fluids such as blood, breast milk, cerebrospinal fluid and urine prior to isolation of EVs. Module 4 and 5 will present different isolation methods and characterization/quantification techniques, respectively. Here differential ultracentrifugation, size exclusion chromatography, density gradient, kit based precipitation, electron microscopy (EM), cryo-TEM, flow cytometry, atomic-force microscopy and nanoparticle tracking analysis will be presented. The recommended prerequisites are college-freshman-level biology and biochemistry. After a completed course you should be able to: + Discuss the nomenclature and subgroups of extracellular vesicles. + Describe the RNA, protein and lipid content of extracellular vesicles. + Describe the basic concepts about the most common isolation and characterization techniques and how these techniques are used in the EV field. + State the benefits and limitations of the most common isolation and characterization techniques for extracellular vesicles. + Explain the considerations that are important during the collection and isolation of EVs from different body fluid. + Describe the release and uptake mechanisms of extracellular vesicles All lectures are given in English. Each of the five modules will be followed by an exam. All exams will be in the format of multiple choice questions. The course is organized in collaboration between the International Society for Extracellular Vesicles (ISEV), University of California Irvine (USA), University of Gothenburg (Sweden) and Pohang University of Science and Technology (South Korea)....
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Intermediate Level

Intermediate Level

Clock

Suggested: 5 weeks, 2-3 hours/week

Approx. 12 hours to complete
Comment Dots

English

Subtitles: English

Skills you will gain

BiologySample PreparationSample CollectionCell Biology
Globe

100% online courses

Start instantly and learn at your own schedule.
Calendar

Flexible deadlines

Reset deadlines in accordance to your schedule.
Intermediate Level

Intermediate Level

Clock

Suggested: 5 weeks, 2-3 hours/week

Approx. 12 hours to complete
Comment Dots

English

Subtitles: English

Syllabus - What you will learn from this course

1

Section
Clock
2 hours to complete

Introduction to the Course and the Field of Extracellular Vesicles

In this module, we will introduce the course and the field of extracellular vesicles. We will discuss the nomenclature and the history of extracellular vesicles as well as the organisms and places that have been shown to release/contain extracellular vesicles. ...
Reading
4 videos (Total 69 min), 1 reading, 1 quiz
Video4 videos
Introduction to the Course11m
The Origin of EVs throughout the Phylogenetic Tree11m
History of Exosomes and Extracellular Vesicles32m
Reading1 reading
Syllabus10m
Quiz1 practice exercise
Introduction to Extracellular Vesicles18m

2

Section
Clock
3 hours to complete

Biogenesis, Cargo and Uptake of Extracellular Vesicles

In this module, we will focus on the biogenesis and release of extracellular vesicles as well as the uptake mechanisms of extracellular vesicles when they are encountered by a recipient cell. This module also covers the different RNA, protein and lipid types present in extracellular vesicles as well as a brief overview what the functions of these molecules are. Furthermore, the techniques that are commonly used to detect these molecules as well as databases that can be useful to use when analyzing the cargo of extracellular vesicles will be highlighted....
Reading
6 videos (Total 142 min), 1 quiz
Video6 videos
Mechanisms of Extracellular Vesicle Uptake - Part 122m
Mechanisms of Extracellular Vesicle Uptake - Part 223m
The Protein Content of Extracellular Vesicles23m
The RNA Content of Extracellular Vesicles35m
The Lipid Content of Extracellular Vesicles18m
Quiz1 practice exercise
Biogenesis, Cargo and Uptake of Extracellular Vesicles30m

3

Section
Clock
2 hours to complete

Collection and Processing of Cell Culture Media and Body Fluids Prior to Isolation of Extracellular Vesicles

In this module, we will focus on the collection and processing of cell culture media and body fluids prior to isolation of extracellular vesicles. We will discuss some of the considerations and guidelines that are important during the collection of staring material and isolation of extracellular vesicles. This module will help you with the different choices, such as anticoagulants, collection time points and protein inhibitors, which are important for the outcome when working with a particular body fluid compared to conditional media or other body fluids. This module will also illustrate some example of studies on extracellular vesicles from body fluid such as blood, urine, breast milk and cerebrospinal fluid and why it is of interest to analyse extracellular vesicles from these body fluids. ...
Reading
5 videos (Total 79 min), 1 quiz
Video5 videos
Blood Plasma and Serum15m
Breast Milk8m
Urine25m
Cerebrospinal Fluid15m
Quiz1 practice exercise
Collection and Processing of Cell Culture Media and Body Fluids Prior to Isolation of Extracellular Vesicles36m

4

Section
Clock
2 hours to complete

Methods for Isolating Extracellular Vesicles

This module highlights some of the different methods used to isolate extracellular vesicles. Here the basic concepts for methods such as differential ultracentrifugation, density gradient, size exclusion and kit-based precipitation will be covered and some guidelines will be presented. Furthermore, this module will cover how the techniques are used in the field of extracellular vesicles as well as their limitations and benefits....
Reading
6 videos (Total 77 min), 1 quiz
Video6 videos
Differential Ultracentrifugation - Part 216m
Density Gradient12m
Size Exclusion Chromatography14m
Kit-based Precipitation12m
Summary of Isolation Methods for Extracellular Vesicles6m
Quiz1 practice exercise
Methods for Isolating Extracellular Vesicles22m

Instructor

Cecilia Lasser

Researcher
Krefting Research Centre, University of Gothenburg

About University of California, Irvine

Since 1965, the University of California, Irvine has combined the strengths of a major research university with the bounty of an incomparable Southern California location. UCI’s unyielding commitment to rigorous academics, cutting-edge research, and leadership and character development makes the campus a driving force for innovation and discovery that serves our local, national and global communities in many ways....

Frequently Asked Questions

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