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Learner Reviews & Feedback for Basics of Extracellular Vesicles by University of California, Irvine

4.8
stars
233 ratings
58 reviews

About the Course

This course aims to provide the basic knowledge about extracellular vesicles (EV) a generic term including exosomes, microvesicles, microparticles, ectosomes, oncosomes, prostasomes, and many others. It covers areas such as EV history, nomenclature, biogenesis, EV cargo as well as the release and uptake mechanisms, collection and processing prior to isolation, different isolation methods, characterization and quantification techniques. This course is divided into five modules. Module 1 is an introduction to the field and will cover the nomenclature and the history of EVs. Module 2 will focus on the biogenesis, release and uptake mechanisms of EVs as well as the different EV cargos (RNA, protein, lipids). In Module 3, we will focus on the collection and processing of cell culture media and body fluids such as blood, breast milk, cerebrospinal fluid and urine prior to isolation of EVs. Module 4 and 5 will present different isolation methods and characterization/quantification techniques, respectively. Here differential ultracentrifugation, size exclusion chromatography, density gradient, kit based precipitation, electron microscopy (EM), cryo-TEM, flow cytometry, atomic-force microscopy and nanoparticle tracking analysis will be presented. The recommended prerequisites are college-freshman-level biology and biochemistry. After a completed course you should be able to: + Discuss the nomenclature and subgroups of extracellular vesicles. + Describe the RNA, protein and lipid content of extracellular vesicles. + Describe the basic concepts about the most common isolation and characterization techniques and how these techniques are used in the EV field. + State the benefits and limitations of the most common isolation and characterization techniques for extracellular vesicles. + Explain the considerations that are important during the collection and isolation of EVs from different body fluid. + Describe the release and uptake mechanisms of extracellular vesicles All lectures are given in English. Each of the five modules will be followed by an exam. All exams will be in the format of multiple choice questions. The course is organized in collaboration between the International Society for Extracellular Vesicles (ISEV), University of California Irvine (USA), University of Gothenburg (Sweden) and Pohang University of Science and Technology (South Korea)....

Top reviews

MH

Jan 11, 2019

Awosome course. Lot of information given with paper based data and suggestion. I really love it. I am also working with Exosome. I use cell culture media from PC12 and fibroblast cell line.

DD

May 17, 2020

I found Ia very professional way to introduce everyone to the amazing world of EV. Professor show elegantly their knowledge. The course fulfils my expectations. Congratulations! Nice works

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26 - 50 of 55 Reviews for Basics of Extracellular Vesicles

By Laura E S S

Aug 05, 2019

Considero que los temas están muy bien resumidos y son fáciles de entender, los tiempos son flexibles.

By Thijs G

Sep 29, 2016

I found this a very informative course on the recent developments and the basics of the EV field.

By Shiyou C

Feb 13, 2017

The course provide a general introduction of this field and the methods usde in the experiments.

By Elizabeth R F

Jul 01, 2019

This course was so amazing! It was so hard but I learned so much! Good job Cecilia Laaser!

By Lorena L R V

May 29, 2017

Excelente curso, reafirma los conceptos básicos, da consejos para las distintas técnicas.

By Francielton d S L

Sep 08, 2019

It was a great experience, i was able to know more about it and apply it to my research.

By Geraldo M J

Jun 23, 2020

Um curso incrível!!!! Aprendi o necessário para o meu trabalho de iniciação científica.

By Thitaporn S

Dec 26, 2019

This course provides enough essential information for study of extracellular vesicles.

By Jess M

Sep 14, 2016

Aprendí mucho. Muy completo, aunque pudieron haber profundizado un poco más.

By LIN, T

Mar 02, 2020

Great course to learn the basic knowledges of extracellular vesicles!

By Asmaa A M A

May 14, 2020

Excellent course, totally enjoyed it and benefited alot from it

By Laura M M E

May 04, 2020

Excelente curso, expositores con amplio conocimiento del tema

By Stephanie B

Oct 05, 2019

Good basic overview for someone starting in their studies

By Wenhao L

Apr 26, 2017

Very wonderful courses! Better than reading 100 papers!

By Mohammad R

Feb 05, 2017

This course gives an excellent overview of the field!

By Nahla I S H

Apr 22, 2020

A very good course, really it was very informative.

By pbmansfield

Mar 06, 2017

Well put together and very informative. Great job!

By Dr.� S R S A P -

Jun 26, 2020

very helpful and nice videos and the contents too

By Yingchao M

Apr 09, 2020

Very good for beginners.

By Abner M N

Nov 22, 2019

Amazing course!

By Adel D

Nov 03, 2016

very good!

By Kaio M R

Oct 10, 2017

ÓTIMO

By Emayamsavitha

Apr 27, 2020

Gud

By PRIYASHREE N

Jul 03, 2020

The way the course was introduced and was taught is really amazing. There were few issues in understanding the audio and there was problem with subtitles as well but still it was a good and amazing experience to meet and listen to researchers from various parts of world. Though it is a blooming field, there was a lot of information in this course. And I gained lot of information from this course. Thanks for every single researcher and professor who are involved in this course. It was a great pleasure to meet and hear your lectures.

By Mainak B

Nov 02, 2019

Excellent content for learning about basic of extracellular vesicles beginners. One thing I would like to point out is it would have been great if the course materials are made available as a pdf version.