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Learner Reviews & Feedback for Basics of Extracellular Vesicles by University of California, Irvine

4.7
stars
303 ratings
82 reviews

About the Course

This course aims to provide the basic knowledge about extracellular vesicles (EV) a generic term including exosomes, microvesicles, microparticles, ectosomes, oncosomes, prostasomes, and many others. It covers areas such as EV history, nomenclature, biogenesis, EV cargo as well as the release and uptake mechanisms, collection and processing prior to isolation, different isolation methods, characterization and quantification techniques. This course is divided into five modules. Module 1 is an introduction to the field and will cover the nomenclature and the history of EVs. Module 2 will focus on the biogenesis, release and uptake mechanisms of EVs as well as the different EV cargos (RNA, protein, lipids). In Module 3, we will focus on the collection and processing of cell culture media and body fluids such as blood, breast milk, cerebrospinal fluid and urine prior to isolation of EVs. Module 4 and 5 will present different isolation methods and characterization/quantification techniques, respectively. Here differential ultracentrifugation, size exclusion chromatography, density gradient, kit based precipitation, electron microscopy (EM), cryo-TEM, flow cytometry, atomic-force microscopy and nanoparticle tracking analysis will be presented. The recommended prerequisites are college-freshman-level biology and biochemistry. After a completed course you should be able to: + Discuss the nomenclature and subgroups of extracellular vesicles. + Describe the RNA, protein and lipid content of extracellular vesicles. + Describe the basic concepts about the most common isolation and characterization techniques and how these techniques are used in the EV field. + State the benefits and limitations of the most common isolation and characterization techniques for extracellular vesicles. + Explain the considerations that are important during the collection and isolation of EVs from different body fluid. + Describe the release and uptake mechanisms of extracellular vesicles All lectures are given in English. Each of the five modules will be followed by an exam. All exams will be in the format of multiple choice questions. The course is organized in collaboration between the International Society for Extracellular Vesicles (ISEV), University of California Irvine (USA), University of Gothenburg (Sweden) and Pohang University of Science and Technology (South Korea)....

Top reviews

MH
Jan 10, 2019

Awosome course. Lot of information given with paper based data and suggestion. I really love it. I am also working with Exosome. I use cell culture media from PC12 and fibroblast cell line.

DD
May 16, 2020

I found Ia very professional way to introduce everyone to the amazing world of EV. Professor show elegantly their knowledge. The course fulfils my expectations. Congratulations! Nice works

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51 - 75 of 79 Reviews for Basics of Extracellular Vesicles

By Mohammad R

Feb 5, 2017

This course gives an excellent overview of the field!

By Nahla I S H

Apr 22, 2020

A very good course, really it was very informative.

By pbmansfield

Mar 6, 2017

Well put together and very informative. Great job!

By Dr.� S R S A P -

Jun 26, 2020

very helpful and nice videos and the contents too

By Ramiro R d l C

Nov 4, 2020

Great! Very informative and I learned a lot.

By Emilio E S

Jul 26, 2021

really good course. I learn a lot about EVs

By Regina C

Dec 29, 2020

Very interesting and simple to understand

By Olivia C

Aug 6, 2020

very useful course, I really enjoy it!

By Yingchao M

Apr 9, 2020

Very good for beginners.

By David H

Sep 1, 2020

excellent course

By Abner M N

Nov 21, 2019

Amazing course!

By Dr T S R

Sep 17, 2020

Super Course

By Adel D

Nov 3, 2016

very good!

By CAROLINA T P

Jul 30, 2021

Excellent

By ganesha p

Sep 16, 2020

Excellent

By Lee-Ann M C

Jul 9, 2021

Usefull

By Kaio M R

Oct 9, 2017

ÓTIMO

By Emayamsavitha

Apr 27, 2020

Gud

By PRIYASHREE N

Jul 3, 2020

The way the course was introduced and was taught is really amazing. There were few issues in understanding the audio and there was problem with subtitles as well but still it was a good and amazing experience to meet and listen to researchers from various parts of world. Though it is a blooming field, there was a lot of information in this course. And I gained lot of information from this course. Thanks for every single researcher and professor who are involved in this course. It was a great pleasure to meet and hear your lectures.

By Arjun J

Sep 7, 2021

Definitely got to learn quite a bit of possibly useful stuff (I'm very new to this) during the course. The quality of lectures varied on a spectrum from average to great, depending on the instructor for each topic. While some of them literally just read off the slides they prepared, others delivered the info with evident passion, which kept you attentive and interested. It's a mixed bag, but don't let that stop you - overall, the course experience was not bad.

By Mainak B

Nov 2, 2019

Excellent content for learning about basic of extracellular vesicles beginners. One thing I would like to point out is it would have been great if the course materials are made available as a pdf version.

By Enrique V O

May 11, 2017

It is good much info about vesicles that you can't get from the books and techniques to study them.

By Shalmali P

Mar 3, 2018

Very nice nd inormative presentations. But few words in many presentations are not audible.

By Marine H

Aug 11, 2020

The last two modules were extremely helpful and a very good opener to this topic.

By David d V L

Mar 29, 2018

Excellents classes I have learned a lot of procotols about EVs