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In diesem Kurs gibt es 7 Module
This course is a follow up course to "Researcher's Guide to Fundamentals of Omic Data" which dives into further detail about DNA informatics methods!
This course is for individuals who:
- Have taken Researcher's Guide to Fundamentals of Omic Data
- Have DNA data and don’t know what to do with it.
- Want a basic overview of their DNA focused data type.
- Want to find resources for processing and interpreting DNA data
What this course will cover:
- Fundamentals of DNA methods.
- Resources you may consider looking into for your own purposes.
- Questions you should ask yourself and your colleagues about your goals and experimental design.
What this course will NOT cover:
- Code needed to process your data.
- Details about every type of experimental design.
- Everything you’d need to know to make you a computational biologist. In other words we still highly encourage you to consult your informatics and computational colleagues, especially those who may have done any handling of data you are trying to learn about.
In this module we discuss what this course covers and encourage you to make your own goals for what you'd like to learn. Then we start with some generalities about DNA focused methods.
Das ist alles enthalten
3 Videos3 Lektüren2 Aufgaben1 Diskussionsthema
Infos zu Modulinhalt anzeigen
3 Videos•Insgesamt 6 Minuten
Welcome!•1 Minute
Intro to this course•1 Minute
DNA Overview•4 Minuten
3 Lektüren•Insgesamt 19 Minuten
Considerations for your DNA goals•5 Minuten
Video covering types of mutations•2 Minuten
DNA Overview•12 Minuten
2 Aufgaben•Insgesamt 11 Minuten
DNA Overview•6 Minuten
DNA Overview•5 Minuten
1 Diskussionsthema•Insgesamt 15 Minuten
Goals for this course•15 Minuten
WGS and WXS
Modul 2•1 Stunde abzuschließen
Moduldetails
In this module we cover the details of whole genome sequencing and whole exome sequencing. We compare and contrast the methods and discuss what kinds of questions these data types might answer.
Das ist alles enthalten
2 Lektüren2 Aufgaben1 Diskussionsthema
Infos zu Modulinhalt anzeigen
2 Lektüren•Insgesamt 20 Minuten
DNA Sequencing Video•5 Minuten
WGS and WXS•15 Minuten
2 Aufgaben•Insgesamt 13 Minuten
WGS vs WXS Quiz•8 Minuten
WGS vs WXS•5 Minuten
1 Diskussionsthema•Insgesamt 10 Minuten
Imagine a WXS/WGS study•10 Minuten
DNA Methylation
Modul 3•1 Stunde abzuschließen
Moduldetails
In this section we dive into DNA methylation sequencing methods, also known more specifically as bisulfite sequencing.
Das ist alles enthalten
2 Lektüren2 Aufgaben1 Diskussionsthema
Infos zu Modulinhalt anzeigen
2 Lektüren•Insgesamt 23 Minuten
Video on basics of DNA Methylation•8 Minuten
DNA Methylation•15 Minuten
2 Aufgaben•Insgesamt 13 Minuten
DNA methylation quiz•8 Minuten
DNA Methylation•5 Minuten
1 Diskussionsthema•Insgesamt 10 Minuten
Imagine a DNA methylation study•10 Minuten
Overview of Chromatin
Modul 4•1 Stunde abzuschließen
Moduldetails
In this section we cover the basics of chromatin evaluation methods. We contrast and compare chromatin sequencing methods.
Das ist alles enthalten
1 Video2 Lektüren2 Aufgaben1 Diskussionsthema
Infos zu Modulinhalt anzeigen
1 Video•Insgesamt 2 Minuten
Chromatin Overview•2 Minuten
2 Lektüren•Insgesamt 15 Minuten
Chromatin Methods Overview•10 Minuten
Video about the basics of chromatin•5 Minuten
2 Aufgaben•Insgesamt 13 Minuten
Chromatin Overview Quiz•8 Minuten
Chromatin Overview•5 Minuten
1 Diskussionsthema•Insgesamt 10 Minuten
Imagine a chromatin study•10 Minuten
Chromatin Methods part 1
Modul 5•1 Stunde abzuschließen
Moduldetails
In this module, we discuss details of the different ways chromatin can be assessed. We go through the basics of ATAC-seq, and scRNA-seq.
Das ist alles enthalten
2 Videos3 Lektüren2 Aufgaben
Infos zu Modulinhalt anzeigen
2 Videos•Insgesamt 4 Minuten
ATAC-seq Overview•2 Minuten
Single cell ATAC-seq Overview•3 Minuten
3 Lektüren•Insgesamt 38 Minuten
Video about Basics of ATAC-seq •10 Minuten
ATAC-Seq•15 Minuten
scATAC-Seq•13 Minuten
2 Aufgaben•Insgesamt 13 Minuten
ATAC-seq and scATAC-seq•8 Minuten
ATAC-seq and scATAC-seq•5 Minuten
Chromatin Methods part 2
Modul 6•1 Stunde abzuschließen
Moduldetails
In this section we cover the basics of ChIP-seq and CUT & Tag and CUT & RUN.
Das ist alles enthalten
1 Video4 Lektüren2 Aufgaben
Infos zu Modulinhalt anzeigen
1 Video•Insgesamt 2 Minuten
ChIP-Seq Overview•2 Minuten
4 Lektüren•Insgesamt 31 Minuten
Video about the Basics of ChIP-seq •6 Minuten
ChIP-seq•10 Minuten
Video: CUT & RUN Basics•5 Minuten
CUT&RUN/Tag•10 Minuten
2 Aufgaben•Insgesamt 13 Minuten
ChIP-seq and CUT & RUN/Tag Quiz•8 Minuten
ChIP-seq and CUT & RUN/Tag•5 Minuten
Microarrays and Wrapping Up
Modul 7•1 Stunde abzuschließen
Moduldetails
In this section, In this module we discuss when microarrays should be used for assaying DNA instead of sequencing methods.
and then we will wrap up this course with a final quiz to assess your absorption of the material. We also point you to our Tool Glossary that has a list of tools you may want to consider for your next steps of your DNA omics study.
Das ist alles enthalten
3 Lektüren1 Aufgabe2 Diskussionsthemen
Infos zu Modulinhalt anzeigen
3 Lektüren•Insgesamt 14 Minuten
Consider Microarrays•8 Minuten
About the authors•2 Minuten
Tool Glossary•4 Minuten
1 Aufgabe•Insgesamt 15 Minuten
Summary of DNA tools Quiz•15 Minuten
2 Diskussionsthemen•Insgesamt 20 Minuten
Are SNP arrays for you?•10 Minuten
Which DNA Tools?•10 Minuten
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Based in Seattle, Fred Hutch is an independent, nonprofit organization and the only National Cancer Institute-designated cancer center in Washington. We have earned a global reputation for our track record of discoveries in cancer, infectious disease and basic research, including important advances in bone marrow transplantation, immunotherapy, HIV/AIDS prevention and COVID-19 vaccines. Fred Hutch operates eight clinical care sites that provide medical oncology, infusion, radiation, proton therapy and related services and has network affiliations with hospitals in four states. Fred Hutch also serves as UW Medicine’s cancer program.
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