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Il y a 7 modules dans ce cours
This course is a follow up course to "Researcher's Guide to Fundamentals of Omic Data" which dives into further detail about DNA informatics methods!
This course is for individuals who:
- Have taken Researcher's Guide to Fundamentals of Omic Data
- Have DNA data and don’t know what to do with it.
- Want a basic overview of their DNA focused data type.
- Want to find resources for processing and interpreting DNA data
What this course will cover:
- Fundamentals of DNA methods.
- Resources you may consider looking into for your own purposes.
- Questions you should ask yourself and your colleagues about your goals and experimental design.
What this course will NOT cover:
- Code needed to process your data.
- Details about every type of experimental design.
- Everything you’d need to know to make you a computational biologist. In other words we still highly encourage you to consult your informatics and computational colleagues, especially those who may have done any handling of data you are trying to learn about.
In this module we discuss what this course covers and encourage you to make your own goals for what you'd like to learn. Then we start with some generalities about DNA focused methods.
Inclus
3 vidéos3 lectures2 devoirs1 sujet de discussion
Afficher les informations sur le contenu du module
3 vidéos•Total 6 minutes
Welcome!•1 minute
Intro to this course•1 minute
DNA Overview•4 minutes
3 lectures•Total 19 minutes
Considerations for your DNA goals•5 minutes
Video covering types of mutations•2 minutes
DNA Overview•12 minutes
2 devoirs•Total 11 minutes
DNA Overview•5 minutes
DNA Overview•6 minutes
1 sujet de discussion•Total 15 minutes
Goals for this course•15 minutes
WGS and WXS
Module 2•1 heure à terminer
Détails du module
In this module we cover the details of whole genome sequencing and whole exome sequencing. We compare and contrast the methods and discuss what kinds of questions these data types might answer.
Inclus
2 lectures2 devoirs1 sujet de discussion
Afficher les informations sur le contenu du module
2 lectures•Total 20 minutes
DNA Sequencing Video•5 minutes
WGS and WXS•15 minutes
2 devoirs•Total 13 minutes
WGS vs WXS•5 minutes
WGS vs WXS Quiz•8 minutes
1 sujet de discussion•Total 10 minutes
Imagine a WXS/WGS study•10 minutes
DNA Methylation
Module 3•1 heure à terminer
Détails du module
In this section we dive into DNA methylation sequencing methods, also known more specifically as bisulfite sequencing.
Inclus
2 lectures2 devoirs1 sujet de discussion
Afficher les informations sur le contenu du module
2 lectures•Total 23 minutes
Video on basics of DNA Methylation•8 minutes
DNA Methylation•15 minutes
2 devoirs•Total 13 minutes
DNA Methylation•5 minutes
DNA methylation quiz•8 minutes
1 sujet de discussion•Total 10 minutes
Imagine a DNA methylation study•10 minutes
Overview of Chromatin
Module 4•1 heure à terminer
Détails du module
In this section we cover the basics of chromatin evaluation methods. We contrast and compare chromatin sequencing methods.
Inclus
1 vidéo2 lectures2 devoirs1 sujet de discussion
Afficher les informations sur le contenu du module
1 vidéo•Total 2 minutes
Chromatin Overview•2 minutes
2 lectures•Total 15 minutes
Chromatin Methods Overview•10 minutes
Video about the basics of chromatin•5 minutes
2 devoirs•Total 13 minutes
Chromatin Overview•5 minutes
Chromatin Overview Quiz•8 minutes
1 sujet de discussion•Total 10 minutes
Imagine a chromatin study•10 minutes
Chromatin Methods part 1
Module 5•1 heure à terminer
Détails du module
In this module, we discuss details of the different ways chromatin can be assessed. We go through the basics of ATAC-seq, and scRNA-seq.
Inclus
2 vidéos3 lectures2 devoirs
Afficher les informations sur le contenu du module
2 vidéos•Total 4 minutes
ATAC-seq Overview•2 minutes
Single cell ATAC-seq Overview•3 minutes
3 lectures•Total 38 minutes
Video about Basics of ATAC-seq •10 minutes
ATAC-Seq•15 minutes
scATAC-Seq•13 minutes
2 devoirs•Total 13 minutes
ATAC-seq and scATAC-seq•5 minutes
ATAC-seq and scATAC-seq•8 minutes
Chromatin Methods part 2
Module 6•1 heure à terminer
Détails du module
In this section we cover the basics of ChIP-seq and CUT & Tag and CUT & RUN.
Inclus
1 vidéo4 lectures2 devoirs
Afficher les informations sur le contenu du module
1 vidéo•Total 2 minutes
ChIP-Seq Overview•2 minutes
4 lectures•Total 31 minutes
Video about the Basics of ChIP-seq •6 minutes
ChIP-seq•10 minutes
Video: CUT & RUN Basics•5 minutes
CUT&RUN/Tag•10 minutes
2 devoirs•Total 13 minutes
ChIP-seq and CUT & RUN/Tag•5 minutes
ChIP-seq and CUT & RUN/Tag Quiz•8 minutes
Microarrays and Wrapping Up
Module 7•1 heure à terminer
Détails du module
In this section, In this module we discuss when microarrays should be used for assaying DNA instead of sequencing methods.
and then we will wrap up this course with a final quiz to assess your absorption of the material. We also point you to our Tool Glossary that has a list of tools you may want to consider for your next steps of your DNA omics study.
Inclus
3 lectures1 devoir2 sujets de discussion
Afficher les informations sur le contenu du module
3 lectures•Total 14 minutes
Consider Microarrays•8 minutes
About the authors•2 minutes
Tool Glossary•4 minutes
1 devoir•Total 15 minutes
Summary of DNA tools Quiz•15 minutes
2 sujets de discussion•Total 20 minutes
Are SNP arrays for you?•10 minutes
Which DNA Tools?•10 minutes
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Based in Seattle, Fred Hutch is an independent, nonprofit organization and the only National Cancer Institute-designated cancer center in Washington. We have earned a global reputation for our track record of discoveries in cancer, infectious disease and basic research, including important advances in bone marrow transplantation, immunotherapy, HIV/AIDS prevention and COVID-19 vaccines. Fred Hutch operates eight clinical care sites that provide medical oncology, infusion, radiation, proton therapy and related services and has network affiliations with hospitals in four states. Fred Hutch also serves as UW Medicine’s cancer program.
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