About this Course
4.7
181 ratings
36 reviews
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100% online

Start instantly and learn at your own schedule.
Flexible deadlines

Flexible deadlines

Reset deadlines in accordance to your schedule.
Beginner Level

Beginner Level

Hours to complete

Approx. 11 hours to complete

Suggested: 12-18 hours of videos and labs...
Available languages

English

Subtitles: English
100% online

100% online

Start instantly and learn at your own schedule.
Flexible deadlines

Flexible deadlines

Reset deadlines in accordance to your schedule.
Beginner Level

Beginner Level

Hours to complete

Approx. 11 hours to complete

Suggested: 12-18 hours of videos and labs...
Available languages

English

Subtitles: English

Syllabus - What you will learn from this course

Week
1
Hours to complete
3 hours to complete

Protein Motifs

In this module we'll be exploring conserved regions within protein families. Such regions can help us understand the biology of a sequence, in that they are likely important for biological function, and also be used to help ascribe function to sequences where we can't identify any homologs in the databases. There are various ways of describing the conserved regions from simple regular expressions to profiles to profile hidden Markov models (HMMs)....
Reading
4 videos (Total 34 min), 4 readings, 1 quiz
Video4 videos
Lecture21m
Lab Discussion9m
Summary1m
Reading4 readings
Acknowledgements10m
Course Logistics10m
Lecture Materials10m
Lab 1 -- Protein Domain, Motif and Profile Analysis30m
Quiz1 practice exercise
Lab 1 Quiz6m
Week
2
Hours to complete
2 hours to complete

Protein-Protein Interactions

In this module we'll be exploring protein-protein interactions (PPIs). Protein-protein interactions are important as proteins don't act in isolation, and often an examination of the interaction partners (determined in an unbiased, perhaps high throughput way) of a given protein can tell us a lot about its biology. We'll talk about some different methods used to determine PPIs and go over their strengths and weaknesses. In the lab we'll use 3 different tools and two different databases to examine interaction partners of BRCA2, a protein that we examined in last module's lab. Finally, we'll touch on a "foundational" concept, Gene Ontology (GO) term enrichment analysis, to help us understand in an overview way the proteins interacting with our example....
Reading
4 videos (Total 37 min), 2 readings, 1 quiz
Video4 videos
Lecture23m
Lab Discussion11m
Summary1m
Reading2 readings
Lecture Materials10m
Lab 2 -- Protein-Protein Interactions30m
Quiz1 practice exercise
Lab 2 Quiz6m
Week
3
Hours to complete
2 hours to complete

Protein Structure

The determination of a protein's tertiary structure in three dimensions can tell us a lot about the biology of that protein. In this module's mini-lecture, we'll talk about some different methods used to determine a protein's tertiary structure and cover the main database for protein structure data, the PDB. In the lab we'll explore the PDB and an online tool for searching for structural (as opposed to sequence) similarity, VAST. We'll then use a nice piece of stand-alone software, PyMOL, to explore several protein structures in more detail....
Reading
4 videos (Total 32 min), 2 readings, 1 quiz
Video4 videos
Lecture13m
Lab Discussion16m
Summary1m
Reading2 readings
Lecture Materials10m
Lab 3 -- Structural Bioinformatics30m
Quiz1 practice exercise
Lab 3 Quiz6m
Week
4
Hours to complete
10 minutes to complete

Review: Protein Motifs, Protein-Protein Interactions, and Protein Structure

...
Reading
1 quiz
Quiz1 practice exercise
Quiz: Protein Motifs, Protein-Protein Interactions, and Protein Structure10m
4.7
36 ReviewsChevron Right
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25%

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Career Benefit

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Career promotion

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Top Reviews

By RGApr 6th 2017

Gives the student real world exposure to the tools to study proteins gene regulation, etc. Instructor is involved and friendly. Highly recommended for someone who is interested in contemporary

By NMJan 9th 2018

Hi Nicholas, Thank you so much for giving a lot of information. Bioinformatic Methods II was little difficult but understood after repeating the lad discussions. Thanks a lot.

Instructor

Avatar

Nicholas James Provart

Professor
Cell & Systems Biology

About University of Toronto

Established in 1827, the University of Toronto is one of the world’s leading universities, renowned for its excellence in teaching, research, innovation and entrepreneurship, as well as its impact on economic prosperity and social well-being around the globe. ...

Frequently Asked Questions

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  • When you purchase a Certificate you get access to all course materials, including graded assignments. Upon completing the course, your electronic Certificate will be added to your Accomplishments page - from there, you can print your Certificate or add it to your LinkedIn profile. If you only want to read and view the course content, you can audit the course for free.

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