Are you a living creature? Then, congratulations! You’ve got DNA. But how much do you really know about the microscopic molecules that make you unique?
Why is DNA called the “blueprint of life”?
What is a “DNA fingerprint”?
How do scientists clone DNA?
What can DNA teach you about your family history?
Are Genetically Modified Organisms (GMOs) safe?
Is it possible to revive dinosaurs by cloning their DNA?
DNA Decoded answers these questions and more. If you’re curious about DNA, join Felicia Vulcu and Caitlin Mullarkey, two biochemists from McMaster University, as they explore the structure of DNA, how scientists cracked the genetic code, and what our DNA can tell us about ourselves. Along the way, you’ll learn about the practical techniques that scientists use to analyze our genetic risks, to manipulate DNA, and to develop new treatments for a range of different diseases. Then, step into our virtual lab to perform your own forensic DNA analysis of samples from a crime scene and solve a murder.
In this module, we'll explore the molecular structure of DNA. What is DNA? What are the basic building blocks of DNA? Where can you find DNA within a cell? We'll learn about how James Watson and Francis Crick were able to solve the riddle of the molecular structure of DNA by building on the work of other scientists. Their groundbreaking discovery revealed that four nucleobases (adenine, thymine, cytosine, and guanine) combine with sugar and phosphate molecules to form the familiar double helix of DNA. We'll take a look at how the molecular structure of DNA regulates its functions; for example, how the chemical bonds (covalent and noncovalent) between these molecules allow DNA to "unzip" during replication. Then we'll take a look at how you manage to fit over three billion base pairs into each of your cells. (Here's a hint: Histones, nucleosomes, and chromosomes would be great at packing for a trip!)
Behind the Picture: Photo 51 (interview)•10 minutes
(Not Really) The Last Word about DNA (video)•10 minutes
Biochemistry: The Operating System of Life (video)•10 minutes
6 assignments•Total 180 minutes
Cracking the Genetic Code•30 minutes
How to Speak DNA•30 minutes
Cracking the Genetic Code•30 minutes
The Building Blocks of DNA•30 minutes
Packing it all in!•30 minutes
DNA Word Search•30 minutes
4 discussion prompts•Total 40 minutes
Why are you interested in learning about DNA?•10 minutes
What's your inspiration?•10 minutes
What are you curious about?•10 minutes
What was the muddiest point in this lesson for you?•10 minutes
Getting the Message Across: Transcription, Translation, and Replication
Module 2•6 hours to complete
Module details
We've all heard DNA described as "the blueprint of life," but what does that actually mean? Each one of the approximately 20,000 genes in our bodies contains the instructions for building a protein. In this module, we'll explain how RNA copies the genetic information contained in our genes and uses this information to assemble amino acids into proteins. Scientists call this concept "Central Dogma": DNA makes RNA and RNA makes protein. We'll also explore how we transmit genetic information from one cell to another -- and what happens when things go wrong. Along the way, we'll learn about techniques (such as polymerase chain reactions and gel electrophoresis) that forensic scientists use in DNA fingerprinting. Join us this week for murder and mayhem in the lab!
TED Talk: Drew Barry - Animations of Unseeable Biology (video)•10 minutes
Protein Data Bank•10 minutes
9 assignments•Total 270 minutes
Getting the Message Across•30 minutes
The Scene of the Crime•30 minutes
Stop copying me! DNA Replication•30 minutes
Central Dogma, Pt 1: Transcription•30 minutes
Central Dogma, Pt 2: Translation•30 minutes
Decipher the Secret Message•30 minutes
Breaking the Code (aka Game of Codons)•30 minutes
Polymerase Chain Reaction (PCR)•30 minutes
Gel Electrophoresis•30 minutes
2 discussion prompts•Total 20 minutes
What are you curious about?•10 minutes
What was the muddiest point in this lesson for you?•10 minutes
Manipulating DNA
Module 3•6 hours to complete
Module details
In this module, we'll explore the techniques scientists use to manipulate DNA. Genetic engineering has allowed us to increase crop yields, diagnose illnesses, develop vaccines, and manufacture insulin. By carefully selecting a pair of molecular scissors (restriction enzymes), scientists are able to isolate a gene of interest and insert it into plasmid DNA, turning a common bacteria (E. coli) into a molecular copying machine. This week, we'll learn that mutations are genetic errors, but that not all mutations are necessarily bad — in fact, some mutations confer a selective advantage that protects against disease. We'll explain what genetically modified organisms are (and what they are not) and weigh in on the heated debates about the ethics and safety of GMOs in popular media.
From Bananas to Papayas: Genetically Modified Organisms (GMOs)•9 minutes
Summing Up•4 minutes
Genetic Engineering•1 minute
Sickle Cell Anemia•1 minute
5 readings•Total 50 minutes
Labster: Molecular Cloning Simulation•10 minutes
Creating Dolly (video)•10 minutes
Genetic Engineering and Working with DNA (video)•10 minutes
How did they make insulin from recombinant DNA? (article)•10 minutes
Massive Review Reveals Consensus on GMO Safety (article)•10 minutes
7 assignments•Total 210 minutes
Manipulating DNA•30 minutes
Freaky Fruit: Manipulating DNA•30 minutes
Slice and Dice: Restriction Enzymes•30 minutes
Lights, Camera, Transformations•30 minutes
Mutations, mutations, mutations•30 minutes
From Papayas to Bananas: Genetically Modified Organisms (GMOs)•30 minutes
Help Felicia design a blue tomato!•30 minutes
4 discussion prompts•Total 40 minutes
If you could manipulate DNA, what would you change?•10 minutes
Should genetically-modified foods be labelled?•10 minutes
What are you curious about?•10 minutes
What was the muddiest point in this lesson for you?•10 minutes
DNA and Me
Module 4•5 hours to complete
Module details
What does your DNA say about you? You may have heard that you share 99% of your genetic material with everyone else on the planet. That's true, but this week we're going to take a look at the less than 1% that makes you unique. We'll kick off by discussing the Human Genome Project, the massive scientific collaboration that mapped out the sequence of all our genetic material. The technologies developed while mapping out the human genome ushered in a new age in DNA research. Now, genome-wide association studies can analyze massive amounts of data, searching for genetic variations that are associated with particular diseases. Pharmacogenomics may help determine which drugs are likely be most effective for you. Genetic genealogy tests (such as Ancestry DNA, 23 and Me, and Family Tree DNA) will allow you to trace random mutations in your DNA that can provide clues to your ethnic heritage. As an added bonus, we'll discuss whether it would be possible to revive extinct species by studying ancient DNA.
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Reviewed on May 29, 2019
I have taken several courses on coursera but I never forget this one. This is the only course my six years old boy enjoy the videos more than me. Thanks for a very educative and interactive videos.
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5·
Reviewed on Jan 25, 2023
Dr. Felicia and Dr. Caitlin are really amazing scientists! Their efforts for this course were truly appreciated. I love their energy, and the knowledge they tried to put up was wonderful.
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Reviewed on Aug 30, 2020
Dr. Felicia and Dr. Caitlin were really engaging and they have sense of humor which I am really looking forward in a course. All in all, it was brief yet informative. Thank you!
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