The course introduces the three key spectroscopic methods used by chemists and biochemists to analyse the molecular and electronic structure of atoms and molecules. These are UV/Visible , Infra-red (IR) and Nuclear Magnetic Resonance (NMR) spectroscopies. The content is presented using short focussed and interactive screencast presentations accompanied by formative quizzes to probe understanding of the key concepts presented. Numerous exercises are provided to facilitate mastery of each topic. A unique virtual spectroscopic laboratory is made available to enable students to measure and analyse spectra online. Assessment is via summative quizzes completed during the course period.
In this first week we introduce the electromagnetic spectrum and the origin of transitions giving rise to ultraviolet and visible (UV/Vis) spectra. You will learn that electronic transitions are caused by absorption of radiation in the UV/Vis region of the electromagnetic spectrum. The reason for the wavelength and intensity of bands will be described and the colour origin of certain compounds will be discussed. You will also be shown how UV/Vis spectroscopy is performed and you will be able to run and analyse your own spectra. As the final activity in this module you are given a link to view how to obtain a UV/Visible spectrum in the laboratory. Good luck, try and participate in the discussion forums to enhance your learning and don't forget to complete the end of week laboratory quiz which contributes to your final mark.
What's included
10 videos7 readings2 assignments
Show info about module content
10 videos•Total 89 minutes
Introduction•2 minutes
What is spectroscopy?•10 minutes
The nature and properties of electromagnetic radiation•7 minutes
Example of energy calculation•11 minutes
Energy levels: Molecular Orbital Theory - revision•11 minutes
Molecular Orbital diagram for CO•5 minutes
UV/Visible spectroscopy•11 minutes
Example of Beer-Lambert calculation•9 minutes
Transitions relevant to UV/Vis Spectroscopy•15 minutes
Effect of conjugation on wavelength•9 minutes
7 readings•Total 65 minutes
Guidance for studying the course•10 minutes
ACKNOWLEDGEMENTS•10 minutes
Pre-course survey•5 minutes
Week 1 Lecture Notes•10 minutes
Lab 1 Introduction•10 minutes
How to obtain a UV/Vis spectrum•10 minutes
Run a UV/Visible spectrum•10 minutes
2 assignments•Total 60 minutes
UV/Vis laboratory quiz•30 minutes
Week 1•30 minutes
Infrared Spectroscopy
Module 2•3 hours to complete
Module details
In this module we introduce the theory underpinning infrared (IR) spectroscopy and show examples of analysis using the technique. Transitions between the vibrational energy levels of molecules occurs in the infrared region of the electromagnetic spectrum. We start with the theory underlying vibration using the simple harmonic oscillator model. Analysis of more complex molecules is introduced using group frequencies and number of vibrational modes. You will also be shown how to obtain an infrared spectrum and will have an opportunity to run your own spectrum. At the end of this module you are given a link to view how to obtain an infra red spectrum in the laboratory. Don't forget to complete the end of week laboratory quiz which contributes to your final mark for this course.
What's included
6 videos4 readings2 assignments
Show info about module content
6 videos•Total 72 minutes
Infrared spectroscopy•13 minutes
Vibrational frequency•12 minutes
Example vibrational frequency calculation•10 minutes
Energy levels and dipoles•15 minutes
Vibrational modes•10 minutes
Example IR spectra of organic molecules•12 minutes
4 readings•Total 40 minutes
Week 2 Lecture Notes•10 minutes
Lab 2 Introduction•10 minutes
How to obtain an infra red spectrum•10 minutes
Run an infra red spectrum•10 minutes
2 assignments•Total 60 minutes
IR Lab Quiz•30 minutes
Week 2•30 minutes
Nuclear Magnetic Resonance (NMR) Spectroscopy
Module 3•4 hours to complete
Module details
This week we concentrate on Nuclear Magnetic Resonance (NMR) spectroscopy. Here a magnetic field is used to create energy levels for magnetic nuclei present in a molecule. Transition between these energy levels occurs in the radiofrequency region of the electromagnetic spectrum. The positions of the bands in the observed spectrum is dependent on the shielding of the nuclei by the local electronic structure, giving rise to a parameter known as chemical shift. Bands also display fine structure caused by spin-spin coupling with neighbouring nuclei. Examples on the analysis of NMR spectra for structure determination will be given. As the final activity in this module you are given a link to view how to obtain an NMR spectrum in the laboratory. Don't forget to compete this end of week laboratory quiz which contributes to your final mark.
What's included
9 videos4 readings2 assignments
Show info about module content
9 videos•Total 114 minutes
NMR Spectroscopy Introduction•12 minutes
Nuclear spin in an external magnetic field•11 minutes
Spin and magnetisation•14 minutes
Chemical shift•18 minutes
More on chemical shift•19 minutes
Analysis of proton spectra•5 minutes
Spin-spin coupling•7 minutes
Spin-spin coupling mechanism•18 minutes
Carbon-13 NMR and pulsed methods•11 minutes
4 readings•Total 40 minutes
Week 3 Lecture Notes•10 minutes
Lab 3 Introduction•10 minutes
How to obtain an NMR spectrum•10 minutes
Run an NMR spectrum•10 minutes
2 assignments•Total 60 minutes
NMR lab quiz•30 minutes
Week 3•30 minutes
Final Assessment
Module 4•1 hour to complete
Module details
What's included
1 assignment
Show info about module content
1 assignment•Total 60 minutes
Week 4 Quiz•60 minutes
Instructor
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P
PP
4·
Reviewed on May 19, 2021
it was very useful for the people who are new to this topic. but they do have to include some more small topics like red shift blue shift and emphasis more on the spectral bands.
K
KB
5·
Reviewed on Oct 14, 2020
This course helped me to further understand the principle behinds the 3 main spectroscopic techniques: UV/VIS, IR and NMR which is very beneficial for my future career working in a lab environment.
L
LM
5·
Reviewed on May 17, 2020
Such a nice approach is given through this course. There's balance between videos, practices,and quizzes to make sure you get to know what you are dealing with spectroscopy.
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