Antimicrobial resistance is an increasingly pressing global concern. This course offers unique and comprehensive insights into the challenges posed by AMR, exploring its mechanisms, implications for public health and laboratory methods as well as tools for identification.
Participants gain learning experience on how bacteria become resistant and which mechanisms lead to the outcome of resistance. As part of the course, you will also receive demonstration and training in methods for antimicrobial susceptibility testing (AST) and detection of specific resistance in the microbiological laboratories.
Additionally, it will take you through various techniques employed including culture-based methods such as agar dilution and disk diffusion assays which determine the susceptibility of bacteria to antibiotics. Molecular methods such as polymerase chain reaction (PCR) and whole genome sequencing are also outlined in detail equipping you with basic understanding. These methods provide valuable insights into the prevalence and mechanisms of AMR, aiding in the development of effective treatment strategies and surveillance programs.
In our new version an additional module including detection of specific resistance mechanisms has been incorporated.
This module is a welcome to the course where you can find information on the course structure, the content that you will be introduced to in the next modules, the grading, as well as an introduction to the instructors and the teaching material.
What's included
1 video1 reading
Show info about module content
1 video•Total 10 minutes
Welcome to this course•10 minutes
1 reading•Total 10 minutes
NOTE: course update announcement•10 minutes
Module A- Antimicrobial and Antimicrobial Action
Module 2•1 hour to complete
Module details
In this module you will learn about antibiotics/ antimicrobials and you will get to know more about what they are, where they come from, what are the major groups and how can we classify them, as well as how do they have an effect and how do they act on the bacterial cells.
What's included
2 videos1 assignment
Show info about module content
2 videos•Total 34 minutes
Lecture 1- Antimicrobials and antimicrobial classification•21 minutes
Lecture 2- Antimicrobials and antimicrobial action•13 minutes
This module contains information about antimicrobials and the development of resistance in bacteria including causes for resistance and their mechanisms, as well as an insight in how resistance disseminates and how it can be selected.
What's included
4 videos1 assignment
Show info about module content
4 videos•Total 55 minutes
Lecture 3- Antimicrobials and resistance•18 minutes
Lecture 4- Antimicrobials and resistance- causes and mechanisms•14 minutes
Lecture 5- Antimicrobial resistance- emergence and dissemination•11 minutes
Lecture 6- Antimicrobials and resistance - selection•12 minutes
This module deals with antimicrobial susceptibility testing in the laboratory: its importance and use, descriptions of the methods and their applications and detailed descriptions of the procedures applied for the main methods. Additionally, this module gives practical insight on how to interpret antimicrobial susceptibility results.
Module D- Alternatives to Phenotypic Testing (Whole Genome Analysis Tools)
Module 5•1 hour to complete
Module details
As alternative to the phenotypical methods, the researcher might decide to use genotypically based methods to identify genes of interest. In the lectures of this module we present the ResFinder tool which may be used to find resistance mechanisms ( genes and in the future versions also specific point mutations) from Whole genome sequences of bacteria of interest. The second lecture is a demonstration of the MyDBFinder which can be used to identify genes of interest defined by the user from Whole genome sequence data. DISCLAIMER: There has been a change since the ResFinder video lecture was prepared – and there is now only one ResFinder web-tool available: http://genepi.food.dtu.dk/resfinder . This version of the ResFinder automatically includes both the ResFinder and PointFinder databases .
What's included
2 videos1 assignment
Show info about module content
2 videos•Total 27 minutes
Lecture 16- Alternative tools for AMR determination- ResFinder tool principles and applications•17 minutes
Lecture 15- My Database Finder (MyDbFinder) tool principles and applications•10 minutes
1 assignment•Total 30 minutes
Q6- Quizz Lectures 15-16 (WGS tools)•30 minutes
Module E- Quality Management and Quality Control
Module 6•1 hour to complete
Module details
This module is dedicated to Quality management and Quality assurance (QA)/ Quality control (QC) procedures and use of QC strains to assure the quality of results of antimicrobial susceptibility testing: importance, elements and principles.
What's included
2 videos1 assignment
Show info about module content
2 videos•Total 23 minutes
Lecture 23- Quality management in Antimicrobial susceptibility testing•10 minutes
Lecture 24- Quality assurance when doing antimicrobial susceptibility testing•13 minutes
Module F- Presentation of Critically Important Antimicrobials
Module 7•2 hours to complete
Module details
The module provides an in-depth understanding of critical important antimicrobials (CIA). You will explore the importance of CIAs, the mechanism of action and the challenges associated.
What's included
5 videos1 assignment
Show info about module content
5 videos•Total 91 minutes
Beta-lactam resistance in Escherichia coli: ESBL and AmpC•18 minutes
Beta-lactam resistance in Escherichia coli: Carbapenemases•15 minutes
Beta-lactam resistance in staphylococcus aureus: MRSA•31 minutes
Background, detection and characterization of colistin resistance•10 minutes
Lecture 11 -Background, detection and characterization of quinolone resistance•17 minutes
1 assignment•Total 30 minutes
Quiz 7•30 minutes
Module G- Examples of antimicrobial resistance surveillance systems
Module 8•1 hour to complete
Module details
In this module, you will explore AMR integrated surveillance systems at local, regional, and global levels, emphasizing the One Health approach. Specific discussions will include national and regional surveillance initiatives like DANMAP, as well as presentations on FAO InFARM, WHO GLASS, and WHO MIA (CIA).
What's included
6 videos
Show info about module content
6 videos•Total 78 minutes
Surveillance- an important action area to combat antimicrobial resistance•17 minutes
DANMAP: Danish Integrated Antimicrobial Monitoring and Research Programme•20 minutes
The InFARM IT platform and system•16 minutes
GLASS: Global Antimicrobial Resistance and Use Surveillance System•11 minutes
WHO Medically Important Antimicrobial list•11 minutes
Closing remarks to course completion•3 minutes
Instructor
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DTU focuses on research in technical and natural sciences that contributes to the development of society. As an industrially orientated university, our goal is to supply high-level international research based on combining theory with constructing models and empirical methods.
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Learner reviews
4.7
2,579 reviews
5 stars
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20.89%
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K
KD
5·
Reviewed on Aug 30, 2020
I would personally recommend this course to any student aiming for a brief introduction to antimicrobial resistance and also to anyone looking for a very nice and quick brushup of the concepts.
H
HA
5·
Reviewed on Feb 17, 2023
This course online course provides information which is not available on any others plate form, this COURSERA plate is much better than any other plate form. I'm impressed by this course.
D
DG
4·
Reviewed on Feb 16, 2021
An informative course especially for identifying drug-resistant strains such as MRSA, ESBLs, et. Also the criteria for avoiding false positives or negatives have been emphasized very clearly.
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